PRMT7 Antibody

• Description:

  Arginine methylation is an irreversible protein modification catalyzed by Arginine methyltransferases, such as PRMT7, which uses S-adenosylmethionine (AdoMet) as the methyl donor. Arginine methylation is implicated in signal transduction, RNA transport and RNA splicing. PRMT7 has two methyltransferase domains, each containing a putative AdoMet-binding motif. The N-terminal methyltransferase domain closely resembles the catalytic core of PRMT5, and the C-terminal domain is most similar to that of PRMT1. Three PRMT7 splice variants have been identified by database analysis. PRMT7 is localized to the nucleus and cytoplasm and moderate expression is observed in adult brain and lung tissues.

• Specifications:

  Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL

• UniProt:

  Q9NVM4

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human protein was used as the immunogen for the PRMT7 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  PCRP-PRMT7-1A4

• Applications:

  FACS, IF

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the PRMT7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This PRMT7 antibody is available for research use only.

• Storage Conditions:

  Aliquot the PRMT7 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the PRMT7 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nucleus, Cytoplasm

• Image Legend:

  Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using PRMT7 antibody (clone PCRP-PRMT7-1A4) . These results demonstrate the foremost specificity of the PCRP-PRMT7-1A4 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.