PREX2 Antibody / DEPDC2

Phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2, also called DEPDC2 (DEP domain-containing protein 2), is a protein that in humans is encoded by the PREX2 gene. The protein encoded by this gene belongs to the phosphatidylinositol 3,4,5-trisphosphate (PIP3) -dependent Rac exchanger (PREX) family, which are Dbl-type guanine-nucleotide exchange factors for Rac family small G proteins. Structural domains of this protein include the catalytic diffuse B-cell lymphoma homology and pleckstrin homology (DHPH) domain, two disheveled, EGL-10, and pleckstrin homology (DEP) domains, two PDZ domains, and a C-terminal inositol polyphosphate-4 phosphatase (IP4P) domain that is found in one of the isoforms. This protein facilitates the exchange of GDP for GTP on Rac1, allowing the GTP-bound Rac1 to activate downstream effectors. Studies also show that the pleckstrin homology domain of this protein interacts with the phosphatase and tensin homolog (PTEN) gene product to inhibit PTEN phosphatase activity, thus activating the phosphoinositide-3 kinase (PI3K) signaling pathway. Conversely, the PTEN gene product has also been shown to inhibit the GEF activity of this protein. This gene plays a role in insulin-signaling pathways, and either mutations or overexpression of this gene have been observed in some cancers.

9007-83-4

Western blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

Q70Z35

Human, Mouse

E. coli-derived recombinant human protein (amino acids D582-Q1094) was used as the immunogen for the PREX2 antibody.

WB, Direct ELISA

Antigen affinity purified

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

After reconstitution, the PREX2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

This PREX2 antibody is available for research use only.

After reconstitution, the PREX2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the PREX2 antibody should be determined by the researcher.