Products for Research Use Only

DEPTOR Antibody / DEPDC6

CAT: 0800-FY12970Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-FY12970Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Specifications
Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL
UniProt
Q8TB45
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
E.coli-derived human DEPDC6/DEPTOR recombinant protein (Position: A22-C409) was used as the immunogen for the DEPTOR antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC, FACS, ELISA
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This DEPTOR antibody is available for research use only.
Storage Conditions
After reconstitution, the DEPTOR antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the DEPTOR antibody should be determined by the researcher.
Location
Cytoplasm (lysosome)
Image Legend
Immunohistochemical staining of DEPDC6/DEPTOR using anti-DEPTOR antibody. DEPDC6/DEPTOR was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-DEPTOR antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.

UniProtKB · Q8TB45

DEP domain-containing mTOR-interacting protein

DPTOR_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q8TB45
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
DEPTOR
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
hDEPTOR
EC number
—
Processing
—
Secondary accessions
B2RCL9, B4DN97, E7EV87, Q96EQ1, Q9H0R7, Q9H894, Q9HA07
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Ligand

Lipid-binding

Cellular component

LysosomeMembrane

Domain

Repeat