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RAD51C Antibody

CAT: 0800-RQ7737Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ7737Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
RAD51 homolog C (S. cerevisiae), also known as RAD51C, is a protein which in humans is encoded by the RAD51C gene. This gene is a member of the RAD51 family of related genes, which encode strand-transfer proteins thought to be involved in recombinational repair of damaged DNA and in meiotic recombination. Ana this gene product interacts with two other DNA repair proteins, encoded by RAD51B and XRCC3, but not with itself. The protein copurifies with XRCC3 protein in a complex, reflecting their endogenous association and suggesting a cooperative role during recombinational repair. This gene is one of four localized to a region of chromosome 17q23 where amplification occurs frequently in breast tumors. Overexpression of the four genes during amplification has been observed and suggests a possible role in tumor progression. Alternative splicing has been observed for this gene and two variants encoding different isoforms have been identified.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
O43502
Host
Rabbit
Reactivity
Human, Rat
Immunogen
E. coli-derived recombinant human protein (amino acids Q11-K342) was used as the immunogen for the RAD51C antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the RAD51C antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This RAD51C antibody is available for research use only.
Storage Conditions
After reconstitution, the RAD51C antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the RAD51C antibody should be determined by the researcher.
Location
Nuclear, cytoplasmic
Image Legend
IHC staining of FFPE human testicular seminoma tissue with RAD51C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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