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RAD51 Antibody

CAT: 0800-V7333IHC-7MLSize: 7 mLDry Ice: NoHazardous: No
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CAT#:0800-V7333IHC-7MLSize:7 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [RefSeq]
CAS Number
9007-83-4
Specifications
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
UniProt
Q06609
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 1-134 from the human protein was used as the immunogen for this RAD51 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
RALPA-1
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Reconstitution
Store the RAD51 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This RAD51 antibody is available for research use only.
Storage Conditions
Store the RAD51 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*
Location
Nuclear
Image Legend
IHC staining of FFPE human colon carcinoma with RAD51 antibody (clone RALPA-1) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

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