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CD133 Recombinant Protein

CAT: 0384-NCP0261-01Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0384-NCP0261-01Size:500 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
CD133, also known as Prominin, was first described as a cell surface marker recognized by monoclonal antibody AC133 on putative hematopoietic stem cells. Subsequent cDNA cloning indicated that CD133 is a five-transmembrane protein with a predicated molecular weight of 97 kDa. Due to heavy glycosylation, its apparent molecular weight is 130 kDa as determined by SDS-PAGE analysis. Besides blood stem cells, CD133 is expressed on and used to isolate other stem cells, including cancer stem cells. A deletion mutation in CD133 produces aberrant protein localization and may result in retinal degeneration in humans.
CAS Number
9000-83-3
Swiss Prot
O43490
Modification Site
NdeI-XhoI
Expression System
Pet-22b (+)
Tag
His-tag
Purity
Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE) .
Solubility
PBS, 4M Urea, PH7.4
Molecular Weight
~31kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Host or Source
E.coli
AA Sequence
GGTGCAAATGTGGAAAAACTGATCTGTGAACCGTATACCAGTAAAGAACTGTTCCGCGTTCTGGATACCCCGTATCTGCTGAATGAAGATTGGGAATATTATCTGAGTGGTAAACTGTTCAATAAGAGTAAAATGAAGCTGACCTTCGAACAGGTGTATAGCGATTGTAAAAAAAATCGCGGTACCTATGGCACCCTGCATCTGCAGAATAGCTTCAATATTAGTGAACATCTGAACATTAACGAGCATACCGGCAGTATTAGCAGCGAACTGGAAAGTCTGAAAGTGAATCTGAATATCTTCCTGCTGGGCGCAGCCGGTCGTAAAAATCTGCAGGACTTCGCCGCCTGCGGCATTGATCGTATGAATTATGATAGTTATCTGGCCCAGACCGGCAAAAGTCCGGCCGGCGTTAATCTGCTGAGCTTCGCATACGATCTGGAAGCAAAAGCCAATAGCCTGCCGCCGGGCAATCTGCGCAATAGCCTGAAACGCGATGCCCAGACCATTAAAACCATTCATCAGCAGCGCGTGCTGCCGATTGAACAGAGCCTGAGCACCCTGTATCAGAGCGTTAAAATTCTGCAGCGTACCGGCAATGGCCTGCTGGAACGCGTTACCCGTATTCTGGCCAGCCTGGACTTCGCCCAGAACTTCATTACCAATAATACCAGTAGTGTTATCATCGAAGAAACCAAAAAATACGGCCGTACCATTATTGGCTACTTCGAACATTATCTGCAGTGGATTGAATTCAGTATTAGTGAAAAAGTGGCAAGCTGCAAACCGGTGGCCACCGCCCTGGATACCGCAGTGGATGTGTTCCTGTGTAGCTATATTATTGATCCGCTGAAT

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