CD133 polyclonal antibody

• Background:

  May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617) . Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439) .

• Structure Composition:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Product Name Alternative:

  PROML1; Prominin-1; Antigen AC133; Prominin-like protein 1; CD133

• Swiss Prot:

  O43490

• Reactivity:

  Human, Mouse

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CD133. The exact sequence is proprietary.

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC

• Dilution:

  WB (1/500 - 1/1000), IHC (1/100 - 1/200)

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 221% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 14 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes endogenous levels of CD133 protein.

• Applications Notes:

  Western blot analysis of CD133 expression in K562 (A), SW620 (B), Jurkat (C), mouse kidney (D), mouse lung (E) whole cell lysates.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4