CD133 polyclonal antibody

• Background:

  CD133, also known as Prominin, was first described as a cell surface marker recognized by monoclonal antibody AC133 on putative hematopoietic stem cells. Subsequent cDNA cloning indicated that CD133 is a five-transmembrane protein with a predicted molecular weight of 97 kDa. Due to heavy glycosylation, its apparent molecular weight is 130 kDa as determined by SDS-PAGE analysis. Besides blood stem cells, CD133 is expressed on and used to isolate other stem cells, including cancer stem cells. A deletion mutation in CD133 produces aberrant protein localization and may result in retinal degeneration in humans.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Product Name Alternative:

  Prominin-1; Antigen AC133; Prominin-like protein 1; CD133; PROM1; PROML1; MSTP061

• Swiss Prot:

  O43490

• Reactivity:

  Human

• Immunogen:

  Recombinant protein of human CD133.

• Conjugation:

  Unconjugated

• Applications:

  WB IF

• Dilution:

  WB: 1:1000~1:2000 IF: 1:100~1:500

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 110 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CD133 polyclonal antibody detects endogenous levels of CD133 protein.

• Applications Notes:

  Western blot (WB) analysis of CD133 polyclonal antibody at 1:1000 dilution Lane1:L02 whole cell lysate (30ug) Lane2:HepG2 whole cell lysate (30ug) Lane3:Panc1 whole cell lysate (30ug) Lane4:H1792 whole cell lysate (30ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4