CD31 Recombinant Protein

• Background:

  CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis. CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical or oxidative stress), presumably by Src family members. The tyrosine phosphorylation mediates associations with a number of SH2 domain-containing binding partners such as PI3 kinase, SHIP, PLCγ, and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules.

• Swiss Prot:

  P16284

• Modification Site:

  NdeI-XhoI

• Expression System:

  Pet-22b (+)

• Tag:

  His-tag

• Purity:

  Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE) .

• Solubility:

  PBS, 4M Urea, PH7.4

• Molecular Weight:

  ~63kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Host or Source:

  E.coli

• CAS Number:

  9000-83-3

• AA Sequence:

  CAGGAAAATAGCTTCACCATTAATAGTGTTGATATGAAAAGCCTGCCGGATTGGACCGTTCAGAATGGTAAAAATCTGACCCTGCAGTGCTTCGCAGATGTGAGTACCACCAGTCATGTGAAACCGCAGCATCAGATGCTGTTCTATAAAGATGATGTTCTGTTCTATAACATCAGTAGTATGAAAAGTACCGAAAGCTACTTCATTCCGGAAGTGCGTATCTATGATAGTGGTACCTATAAATGCACCGTGATTGTTAATAATAAGGAAAAAACCACCGCCGAATATCAGGTGCTGGTGGAAGGTGTGCCGAGCCCGCGCGTTACCCTGGATAAAAAAGAAGCAATTCAGGGTGGTATTGTTCGCGTGAATTGCAGCGTGCCGGAAGAAAAAGCACCGATTCACTTCACCATTGAAAAACTGGAACTGAATGAAAAAATGGTTAAGCTGAAACGCGAAAAAAATAGTCGCGATCAGAACTTCGTTATTCTGGAATTCCCGGTGGAAGAACAGGATCGTGTTCTGAGCTTCCGCTGTCAGGCCCGCATTATTAGTGGCATTCACATGCAGACCAGCGAAAGCACCAAAAGTGAACTGGTTACCGTGACCGAAAGCTTCAGTACCCCGAAATTCCATATTAGTCCGACCGGCATGATTATGGAAGGCGCACAGCTGCATATTAAATGTACCATTCAGGTTACCCATCTGGCCCAGGAATTCCCGGAAATTATTATTCAGAAAGATAAGGCCATCGTTGCACATAATCGTCATGGTAATAAAGCCGTGTATAGTGTTATGGCCATGGTTGAACATAGTGGTAATTATACCTGTAAAGTGGAAAGTAGCCGTATTAGCAAAGTTAGTAGTATTGTTGTTAACATCACCGAACTGTTCAGTAAACCGGAACTGGAAAGTAGCTTCACCCATCTGGATCAGGGCGAACGTCTGAATCTGAGTTGCAGTATTCCGGGTGCACCGCCGGCCAACTTCACCATTCAGAAAGAAGATACCATTGTGAGTCAGACCCAGGACTTCACCAAAATTGCAAGTAAAAGCGATAGTGGCACCTATATCTGTACCGCCGGCATTGATAAAGTTGTTAAAAAAAGCAACACCGTTCAGATTGTTGTGTGTGAAATGCTGAGTCAGCCGCGCATTAGCTATGATGCACAGTTCGAAGTTATTAAAGGCCAGACCATTGAAGTTCGCTGTGAAAGTATTAGCGGCACCCTGCCGATTAGTTATCAGCTGCTGAAAACCAGTAAAGTGCTGGAAAATAGCACCAAAAATAGCAATGATCCGGCAGTGTTCAAAGATAATCCGACCGAAGATGTGGAATATCAGTGCGTTGCAGATAATTGTCATAGCCATGCCAAAATGCTGAGCGAAGTTCTGCGTGTTAAAGTGATTGCACCGGTGGATGAAGTGCAGATTAGCATTCTGAGCAGCAAAGTGGTGGAAAGCGGTGAAGATATTGTGCTGCAGTGTGCCGTTAATGAAGGCAGTGGTCCGATTACCTATAAATTCTATCGTGAAAAAGAGGGTAAGCCGTTCTATCAGATGACCAGTAATGCCACCCAGGCCTTCTGGACCAAACAGAAAGCAAGTAAAGAACAGGAAGGTGAATATTATTGTACCGCCTTCAATCGCGCCAATCATGCAAGCAGTGTTCCGCGTAGTAAAATTCTGACCGTTCGTGTGATTCTGGCCCCGTGGAAAAAA