CD31 polyclonal antibody

• Background:

  CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis. CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical or oxidative stress), presumably by Src family members. The tyrosine phosphorylation mediates associations with a number of SH2 domain-containing binding partners such as PI3 kinase, SHIP, PLCγ, and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Product Name Alternative:

  Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD31; PECAM1

• Swiss Prot:

  P16284

• Reactivity:

  Human

• Immunogen:

  Recombinant protein of human CD31.

• Conjugation:

  Unconjugated

• Applications:

  WB IP IF

• Dilution:

  WB: 1:5000~1:10000 IP 1:50 - 1:200 IF 1:100 - 1:500

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 130 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CD31 polyclonal antibody detects endogenous levels of CD31 protein.

• Applications Notes:

  Western blot (WB) analysis of CD31 polyclonal antibody at 1:5000 dilution Lane1:Jurkat whole cell lysate (30ug) Lane2:THP-1 whole cell lysate (30ug) Lane3:A549 whole cell lysate (30ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4