CD1B Recombinant Protein

• Background:

  The CD1 multigene family encodes five forms of the CD1 T cell surface glycoprotein in human, designated CD1A, 1B, 1C, 1D and 1E. CD1, a type 1 membrane protein, has structural similarity to the MHC class I antigen and has been shown to present lipid antigens for recognition by T lymphocytes. CD1 antigens are associated with β-2-Microglobulin and expressed on cortical thymocytes, Langerhans cells, a B cell subset and some dendritic cells. Specifically, CD1A is a marker for Langerhans cell histiocytosis (LCH) and is found on interdigitating cells. Adaptor-protein complexes and CD1-associated chaperones control CD1 trafficking, and the development and activation of CD1-restricted T cells. Constitutive endocytosis of CD1B molecules and the differential sorting of MHC class II from lysosomes separate peptide- and lipid antigen-presenting molecules during dendritic cell maturation. CD1B is also expressed in interdigitating cells. The human CD1 genes are all closely linked in a cluster mapping at chromosome 1q 23.1.

• Swiss Prot:

  P29016

• Modification Site:

  NdeI-XhoI

• Expression System:

  Pet-22b (+)

• Tag:

  His-tag

• Purity:

  Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE) .

• Solubility:

  PBS, 4M Urea, PH7.4

• Molecular Weight:

  ~32kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Host or Source:

  E.coli

• CAS Number:

  9000-83-3

• AA Sequence:

  AGTGAACATGCATTCCAGGGCCCGACCAGCTTCCATGTGATTCAGACCAGTAGCTTCACCAATAGTACCTGGGCACAGACCCAGGGCAGCGGTTGGCTGGATGATCTGCAGATTCATGGTTGGGATAGTGATAGTGGCACCGCCATCTTCCTGAAACCGTGGAGTAAAGGCAACTTCAGCGATAAAGAAGTTGCAGAACTGGAAGAAATCTTCCGTGTGTATATCTTCGGCTTCGCCCGCGAAGTTCAGGACTTCGCCGGCGACTTCCAGATGAAATATCCGTTCGAAATTCAGGGCATTGCCGGCTGTGAACTGCATAGCGGCGGTGCCATTGTTAGCTTCCTGCGCGGCGCCCTGGGCGGTCTTGACTTCCTGAGCGTTAAAAATGCCAGTTGCGTGCCGAGCCCGGAAGGCGGTAGTCGTGCCCAGAAATTCTGCGCCCTGATTATTCAGTATCAGGGTATTATGGAAACCGTTCGCATTCTGCTGTATGAAACCTGTCCGCGTTATCTGCTGGGTGTGCTGAATGCAGGTAAAGCAGATCTGCAGCGTCAGGTTAAACCGGAAGCATGGCTGAGTAGCGGCCCGAGTCCGGGTCCGGGTCGTCTTCAGCTGGTGTGTCATGTGAGTGGCTTCTATCCGAAACCGGTGTGGGTTATGTGGATGCGTGGTGAACAGGAACAGCAGGGCACCCAGCTGGGCGATATTCTGCCGAATGCCAATTGGACCTGGTATCTGCGCGCCACCCTGGATGTGGCCGATGGCGAAGCAGCCGGTCTGAGCTGTCGCGTTAAACATAGCAGCCTGGAAGGCCAGGATATTATTCTGTATTGGCGCAATCCGACCAGTATTGGTAGC