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RecombinantBetacellulin, Human

CAT: 0384-BK0008-01Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0384-BK0008-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Betacellulin (BTC) is a member of the EGF family of growth factors that also includes EGF, TGF-α, Amphiregulin, HB-EGF, Epiregulin, Tomoregulin, Heregulin and Neuregulins. Mature human BTC protein exhibits 80% amino acidsimilarity with mouse BTC protein. BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids, including serum, milk, and colostrum. It is synthesized primarily as a transmembrane precursor, which is then processed to a mature molecule by proteolytic events. BTC signals through the EGF receptor.Recombinant Human Betacellulin (BTC) produced in E.coli is a single non-glycosylated polypeptide chain containing 81 amino acids. A fully biologically active molecule, rhBTC has a molecular mass of 15 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
CAS Number
9000-83-3
Endotoxin
< 0.2 EU/μg, determined by LAL method.
Purity
> 95% as analyzed by SDS-PAGE.
Bioactivity
The ED50 was determined by the dose-dependent stimulation of the proliferation of murine Balb/3T3 cells is < 0.01 ng/ml, corresponding to a specific activity of >1 x 10ˆ8 units/mg.
Reconstitution
Reconstituted in ddH2O or PBS at 100 μg/ml.
Molecular Weight
15 kDa, observed by reducing SDS-PAGE.
Storage Conditions
Lyophilized recombinant human Betacellulin (BTC) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, human Betacellulin (BTC) should be stable up to 1 week at 4°C or up to 2 months at -20°C.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized after extensive dialysis against PBS.
Host or Source
Escherichia coli.
Recommended Usage
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
AA Sequence
MDGNSTRSP ETNGLLCGDP EENCAATTTQ SKRKGHFSRC PKQYKHYCIK GRCRFVVAEQ TPSCVCDEGY IGARCERVDL FY

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