RecombinantAdiponectin, Mouse

• Description:

  Adiponectin is a hormone mainly produced by adipocytes. Adiponectin forms a homotrimer and exists as higher order multimers in vivo. The receptors of Adiponectin are seven transmembrane G protein coupled receptors: Receptor 1 is expressed in skeletal muscle and Receptor 2 in liver. Adiponectin receives a lot of attention because of its anti-diabetic, anti-atherosclerotic, and anti-inflammatory properties. Adiponectin increases the expression of molecules involved in fatty acid transport, combustion of fatty acid, and energy dissipation, and increases insulin sensitivity of the body. Decreased levels of Adiponectin are associated with hypertension, cardiovascular diseases, and metabolic syndromes. Therefore, Adiponectin has promising potential as a pharmacological agent.Recombinant mouse Adiponectin (rmAdiponectin) produced in E.coli is a single non-glycosylated polypeptide chain containing 227 amino acids. A fully biologically active molecule, rmAdiponectin has a molecular mass of 24.6 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

• Endotoxin:

  < 0.2 EU/μg, determined by LAL method.

• Purity:

  > 95% as analyzed by SDS-PAGE and HPLC.

• Bioactivity:

  ED50 < 5 µg/mL, measured by a cell growth inhibitory assay using M1 cells, corresponding to a specific activity of > 2x 10ˆ2 units/mg.

• Reconstitution:

  Reconstituted in ddH2O at 100 μg/mL.

• Molecular Weight:

  24.6 kDa, observed by reducing SDS-PAGE.

• Storage Conditions:

  Lyophilized recombinant mouse Adiponectin (rmAdiponectin) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmAdiponectin remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.

• Appearance:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Formulation:

  Lyophilized after extensive dialysis against PBS.

• Host or Source:

  Escherichia coli.

• Recommended Usage:

  This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

• CAS Number:

  9000-83-3

• AA Sequence:

  VTT TEELAPALVP PPKGTCAGWM AGIPGHPGHN GTPGRDGRDG TPGEKGEKGD AGLLGPKGET GDVGMTGAEG PRGFPGTPGR KGEPGEAAYV YRSAFSVGLE TRVTVPNVPI RFTKIFYNQQ NHYDGSTGKF YCNIPGLYYF SYHITVYMKD VKVSLFKKDK AVLFTYDQYQ EKNVDQASGS VLLHLEVGDQ VWLQVYGDGD HNGLYADNVN DSTFTGFLLY HDTN