PPAT polyclonal antibody

• Background :

  Atase (amidophosphoribosyltransferase), also known as PPAT (phosphoribosyl pyrophosphate (PRPP) amidotransferase), PRAT or GPAT (glutamine phosphoribosyl pyrophosphate amidotransferase), is a ubiquitously expressed N-terminal nucleophile-type glutamine amidotransferase that belongs to the purine/pyrimidine phosphoribosyltransferase family. Existing as a homotetramer, Atase plays an important role in purine metabolism. More specifically, Atase functions as regulatory enzyme and contains one glutamine amidotransferase type-2 domain. Binding a magnesium ion and a 4Fe-4S cluster as cofactors, Atase catalyzes the first step (the rate-limiting step) in the purine nucleotide biosynthesis pathway, a two-step reaction that results in the formation of phosphoribosylamine from PRPP and glutamine. The first step of this reaction is catalyzed by the N-terminal glutaminase domain while the second step is catalyzed by the C-terminal PRTase domain.

• Structure Composition :

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative :

  Amidophosphoribosyltransferase ; Atase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPAT; PPAT; GPAT

• Swiss Prot :

  Q06203

• Reactivity :

  Human, Monkey

• Immunogen :

  Recombinant full length Human PPAT.

• Conjugation :

  Unconjugated

• Applications :

  WB

• Dilution :

  WB 1:500 - 1:2000

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification :

  Unmodification

• Molecular Weight :

  ~ 57 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  PPAT polyclonal antibody detects endogenous levels of PPAT protein.

• Applications Notes :

  WesternBlot (WB) analysis of PPAT polyclonal antibody

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4