PPAT polyclonal antibody

• Background:

  Atase (amidophosphoribosyltransferase), also known as PPAT (phosphoribosyl pyrophosphate (PRPP) amidotransferase), PRAT or GPAT (glutamine phosphoribosyl pyrophosphate amidotransferase), is a ubiquitously expressed N-terminal nucleophile-type glutamine amidotransferase that belongs to the purine/pyrimidine phosphoribosyltransferase family. Existing as a homotetramer, Atase plays an important role in purine metabolism. More specifically, Atase functions as regulatory enzyme and contains one glutamine amidotransferase type-2 domain. Binding a magnesium ion and a 4Fe-4S cluster as cofactors, Atase catalyzes the first step (the rate-limiting step) in the purine nucleotide biosynthesis pathway, a two-step reaction that results in the formation of phosphoribosylamine from PRPP and glutamine. The first step of this reaction is catalyzed by the N-terminal glutaminase domain while the second step is catalyzed by the C-terminal PRTase domain.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Amidophosphoribosyltransferase ; Atase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPAT; PPAT; GPAT

• Swiss Prot:

  Q06203

• Reactivity:

  Human, Monkey

• Immunogen:

  Recombinant full length Human PPAT.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB 1:500 - 1:2000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 57 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  PPAT polyclonal antibody detects endogenous levels of PPAT protein.

• Applications Notes:

  WesternBlot (WB) analysis of PPAT polyclonal antibody

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4