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PPAT polyclonal antibody

CAT: 384-BS8365-01Size: 50 µLDry Ice: NoHazardous: No
50 µLSelected
PRICE
AVAILABILITY
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Atase (amidophosphoribosyltransferase), also known as PPAT (phosphoribosyl pyrophosphate (PRPP) amidotransferase), PRAT or GPAT (glutamine phosphoribosyl pyrophosphate amidotransferase), is a ubiquitously expressed N-terminal nucleophile-type glutamine amidotransferase that belongs to the purine/pyrimidine phosphoribosyltransferase family. Existing as a homotetramer, Atase plays an important role in purine metabolism. More specifically, Atase functions as regulatory enzyme and contains one glutamine amidotransferase type-2 domain. Binding a magnesium ion and a 4Fe-4S cluster as cofactors, Atase catalyzes the first step (the rate-limiting step) in the purine nucleotide biosynthesis pathway, a two-step reaction that results in the formation of phosphoribosylamine from PRPP and glutamine. The first step of this reaction is catalyzed by the N-terminal glutaminase domain while the second step is catalyzed by the C-terminal PRTase domain.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Amidophosphoribosyltransferase ; Atase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPAT; PPAT; GPAT
Swiss Prot
Q06203
Reactivity
Human, Monkey
Immunogen
Recombinant full length Human PPAT.
Conjugation
Unconjugated
Applications
WB
Dilution
WB 1:500 - 1:2000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 57 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
PPAT polyclonal antibody detects endogenous levels of PPAT protein.
Applications Notes
WesternBlot (WB) analysis of PPAT polyclonal antibody
Host or Source
Rabbit

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