RCC2 polyclonal antibody

• Background :

  RCC2/TD-60 is a member of the RCC1 (regulator of chromosome condensation 1) family of guanine nucleotide exchange factors. RCC2/TD-60 is associated with the chromosome passenger complex (CPC), which also consists of aurora B kinase, borealin, INCENP (inner centromere protein) and survivin. The CPC acts at various stages of mitosis, interacts with microtubules and is required for proper chromosome segregation and cytokinesis. Regulation of aurora B kinase is key in the regulation of the CPC. In late mitosis, RCC2/TD-60 is required for spindle assembly and recruitment of survivin and aurora B. RCC2/TD-60 is also required for aurora B activation in vitro and localization of the CPC to centromeres.

• CAS Number :

  9007-83-4

• Structure Composition :

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative :

  Protein RCC2; RCC1-like protein TD-60; Telophase disk protein of 60 kDa; RCC2; KIAA1470; TD60

• Swiss Prot :

  Q9P258

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Synthetic peptide, corresponding to amino acids 470-520 of Human RCC2.

• Conjugation :

  Unconjugated

• Applications :

  WB

• Dilution :

  WB: 1:500~1:1000

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification :

  Unmodification

• Molecular Weight :

  ~ 60 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  RCC2 polyclonal antibody detects endogenous levels of RCC2 protein.

• Applications Notes :

  Western blot (WB) analysis of RCC2 polyclonal antibody at 1:500 dilution Lane1:C6 whole cell lysate (40ug) Lane2:CT26 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:HCT116 whole cell lysate (40ug) Lane5:HepG2 whole cell lysate (40ug)

• Host or Source :

  Rabbit