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WEE2 polyclonal antibody

CAT: 0384-BS61656-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0384-BS61656-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Wee1-like protein kinase 2, Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Wee1-like protein kinase 2; Wee1-like protein kinase 1B; Wee1B kinase; WEE2; WEE1B
Swiss Prot
P0C1S8
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding Human WEE2.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 62 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
WEE2 polyclonal antibody detects endogenous levels of WEE2 protein.
Applications Notes
Western blot (WB) analysis of WEE2 polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:PC3 whole cell lysate (40ug) Lane3:MEF whole cell lysate (40ug) Lane4:The Testis tissue lysate of Rat (40ug)
Host or Source
Rabbit

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