CDK5RAP3 polyclonal antibody

• Background :

  CDK5RAP3 (cyclin dependent kinase 5 regulatory subunit-associated protein 3), also designated C53 or IC53, may be involved in neuronal differentiation, cell proliferation and DNA repair. The role CDK5RAP3 plays in neuronal differentiation may be attributed to its interaction with the Cdk5 activator protein called p35. p35 physically associates with Cdk5 to activate enzymatic activity. Cdk5 activity increases significantly during neuronal differentiation. Upon transfection, CDK5RAP3 is capable of increasing the rate of cell proliferation, suggesting that it may play a role in tumorigenesis. CDK5RAP3 is a regulatory component of the G2/M DNA damage checkpoint in response to genotoxic stress. CDK5RAP3 is expressed in brain, heart, placenta, liver, skeletal muscle, lung, kidney and pancreas and is overexpressed in tumor tissue. Three named isoforms exist for CDK5RAP3 as a result of alternative splicing events. CDK5RAP3 contains two leucine zipper motifs, putative phosphorylation and potential N-myristoylation sites.

• Structure Composition :

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative :

  CDK5 regulatory subunit-associated protein 3; CDK5 activator-binding protein C53; LXXLL/leucine-zipper-containing ARF-binding protein; Protein HSF-27

• Swiss Prot :

  Q96JB5

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Synthetic peptide, corresponding to amino acids 211-255 of Human CDK5RAP3.

• Conjugation :

  Unconjugated

• Applications :

  WB

• Dilution :

  WB: 1:500~1:1000

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE) .

• Modification :

  Unmodification

• Molecular Weight :

  ~ 56 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  CDK5RAP3 polyclonal antibody detects endogenous levels of CDK5RAP3 protein.

• Applications Notes :

  Western blot (WB) analysis of CDK5RAP3 polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:Hela whole cell lysate (40ug) Lane4:The brain tissue lysate of Mouse (40ug) Lane5:The brain tissue lysate of Rat (40ug)

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4