SETMAR polyclonal antibody

• Background:

  SETMAR (SET domain and mariner transposase fusion gene-containing protein), also known as METNASE or Hsmar1, is a ubiquitously expressed fusion protein with histone-lysine N-methyltransferase activity and DNA-binding, DNA-looping and DNA-cleavage activities. Localizing to the nucleus, SETMAR contains one N-terminal SET domain which facilitates the histone-lysine methyltransferase activity (at H3-Lys4 and H3-Lys36) and a C-terminal transposase domain which is responsible for the DNA-binding, -looping and -cleavage activities. Both domains are essential for the proper function of SETMAR. SETMAR specifically functions in DNA repair but, on its own, SETMAR can only bind to 5’-TIR (terminal inverted repeats) in DNA. For interactions with non-TIR DNA, SETMAR (via its SET domain) binds to and forms a stable complex with the pre-mRNA processing protein PRP19. Due to alternative splicing events, two isoforms exist.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Histone-lysine N-methyltransferase SETMAR; SET domain and mariner transposase fusion protein; Metnase; Histone-lysine N-methyltransferase; Transposon Hsmar1 transposase; SETMAR

• Swiss Prot:

  Q53H47

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to amino acids 349-394 of Human SETMAR.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB: 1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 78 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  SETMAR polyclonal antibody detects endogenous levels of SETMAR protein.

• Applications Notes:

  Western blot (WB) analysis of SETMAR polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:PC12 whole cell lysate (40ug) Lane3:NIH-3T3 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4