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ERF polyclonal antibody

CAT: 0384-BS61028-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS61028-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Translation is carried out by the ribosome and several associated protein factors through three consecutive steps: initiation, elongation and termination. Termination of protein synthesis takes place when the ribosomal A site is occupied simultaneously by one of three stop codons and by a class 1 translation termination factor. In eukaryotes, this termination factor is the eukaryotic release factor 1 (eRF1), a protein that promotes hydrolysis of the last peptidyl-tRNA on the ribosome. eRF1 activity is stimulated by the association with the GTP-binding protein eRF3. eRF1 forms a quaternary complex with eRF3, GTP and the ribosome. Subunits of eRF3 are eRF3b and eRF3a, which belong to the GTP-binding elongation factor family and are involved in the regulation of cell growth, specifically via control of translation termination.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
ETS domain-containing transcription factor ERF; Ets2 repressor factor; PE-2; ERF
Swiss Prot
P50548
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues in Human ERF.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 60 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
ERF polyclonal antibody detects endogenous levels of ERF protein.
Applications Notes
Western blot (WB) analysis of ERF polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:RAW264.7 whole cell lysate Lane3:PC12 whole cell lysate
Host or Source
Rabbit

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