DFFB polyclonal antibody

• Background:

  The Ced/ICE or caspase family of cysteine proteases plays a pivotol role in mediating apoptosis through the proteolysis of specific targets. Among the targets are poly (ADP-ribose) polymerase (PARP), Gelsolin, ICAD (inhibitor of CAD, also designated DFF-45) and the nuclear lamins. CAD (caspase-activated deoxyribonuclease), also designated CPAN (caspase-activated nuclease) and DFF40, is a DNase that is responsible for DNA degradation during apoptosis. Caspase-3 acts to dissociate CAD from ICAD, allowing CAD to enter the nucleus and degrade DNA.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  DNA fragmentation factor subunit beta (EC:3.-.-.-) ; Caspase-activated deoxyribonuclease; CAD; Caspase-activated DNase; CPAN; DNA fragmentation factor 40 kDa subunit; DFF-40; DFFB

• Swiss Prot:

  O76075

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthetic peptide corresponding to residues in Human DFFB.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB: 1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 39 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  DFFB polyclonal antibody detects endogenous levels of DFFB protein.

• Applications Notes:

  Western blot (WB) analysis of DFFB polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:MCF-7 whole cell lysate Lane3:H9C2 whole cell lysate Lane4:Raw264.7 whole cell lysate

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4