APOBEC3C polyclonal antibody

APOBEC (apolipoprotein B mRNA editing enzyme, catalytic) proteins inhibit retroviruses by deaminating cytosine residues of viral RNA and DNA. The seven APOBEC3 genes or pseudogenes are found in a cluster thought to result from gene duplication on chromosome 22. Like APOBEC3G, APOBEC3F deaminates deoxycytosine to deoxyuracil in the minus strand of HIV-1 DNA, resulting in G to A hypermutation in the plus strand of DNA. Thus, APOBEC3G and APOBEC3F provide a mechanism for innate immunity to retroviruses, and are also likely contribute to sequence variation observed in many viruses. Viral infectivity factor (Vif) imparts APOBEC3G and APOBEC3F resistance to HIV through impaired translation of their mRNA and accelerated posttranslational degradation of the APOBEC3 proteins by the 26S proteasome. Interestingly, HIV-1 Vif cannot form a complex with APOBEC3G or APOBEC3F of mouse origin as it does with the human protein, and thus mouse APOBEC3G and APOBEC3F function as a potent inhibitors of wildtype HIV-1 replication, where human APOBEC3G and APOBEC3F are only able to inhibit Vif-deficient HIV-1 replication. This implies that induction of APOBEC3G and APOBEC3F activity or a method of blocking their interaction with Vif may provide a method for therapeutic intervention.

9007-83-4

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

DNA dC->dU-editing enzyme APOBEC-3C; A3C; APOBEC1-like; Phorbolin I; APOBEC3C; APOBEC1L; PBI

Q9NRW3

Human, Rat

A synthetic peptide corresponding to residues in Human APOBEC3C

Unconjugated

WB IHC

WB: 1:500~1:1000 IHC: 1:50~1:200

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Unmodification

~ 23 kDa

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.

APOBEC3C polyclonal antibody detects endogenous levels of APOBEC3C protein.

Western blot (WB) analysis of APOBEC3C polyclonal antibody at 1:500 dilution Lane1:HepG2 whole cell lysate Lane2:PC12 whole cell lysate