SCRIB polyclonal antibody

• Background:

  Drosophila melanogaster genes, which are categorized based on the type of protein for which they encode, represent six major classifications, including intracellular signaling proteins, transmembrane proteins, RNA binding proteins, secreted factors, transcription regulators (basic helix-loop-helix, homeodomain containing, zinc finger containing and chromatin associated) and other functional proteins. Morphogenesis and cell differentiation in Drosophila requires accurate control of cell division. Discs large (DLG), Scribble (Scrib) and Lethal giant larvae (LGL) tumor suppressor proteins regulate multiple aspects of neuroblast asymmetric cell division. DLG/Scrib/LGL proteins show apical cortical enrichment at prophase/metaphase and have a uniform cortical distribution. Mutations in the genes encoding multi-PDZ (PSD-95, discs-large and ZO-1) and the leucine-rich-repeat protein Scrib cause aberrant cell shapes and the loss of monolayer organization of embryonic epithelia. The human homolog, hScrib, is intracellularly localized to the vertebrate tight junction, which functions to correctly place adherens junctions. The PDZ domains of Scrib are predicted to bind to the consensus S/TXV at the C-terminus of proteins. PDZ domain proteins have been implicated at several different sites of the protein trafficking pathway, suggesting that Scrib is required for the localization of several epithelial determinants.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Protein scribble homolog; Scribble; hScrib; Protein LAP4; SCRIB; CRIB1; KIAA0147; LAP4; SCRB1; VARTUL

• Swiss Prot:

  Q14160

• Reactivity:

  Human

• Immunogen:

  Synthetic peptide, corresponding Human CELSR3.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB: 1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 240 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  SCRIB polyclonal antibodydetects endogenous levels of SCRIB protein.

• Applications Notes:

  Western blot (WB) analysis of SCRIB polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:HCT116 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4