CD3G polyclonal antibody

• Background:

  The T cell antigen receptor (TCR) recognizesforeign antigens and translates such recognition eventsinto intracellularsignalsthat elicit a change in the cell froma dormant to an activated state.Much of this signaling processcan be attributed to a multisubunitcomplex of proteins that associates directly with the TCR. Thiscomplex has been designated CD3 (cluster of differentiation 3) . It iscomposed of five invariant polypeptide chainsthat associate to form three dimers: a heterodimer of γ and ε chains (γε), a heterodimer of δ and ε chains (δε) and a homodimer of two ζ chains (ζζ) or a heterodimer of ζ and η chains (ζη) . The ζ and η chains are encoded bythe same gene but differ in theircarboxyl-terminal ends due to an alternative splicing event. The γ, ε and δ chains each contain a single copy of a conserved immunoreceptor tyrosine-based activationmotif (ITAM) . In contrast, the ζ chain containsthree consecutive copies of the same motif. Phosphorylated ITAMs act as docking sitesfor protein kinases such as ZAP-70 and Syk and are also capable of regulating theirkinase activity. The crystalstructure of ZAP-70’s SH2 domains bound to the ζ chain ITAMs has been solved.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  T-cell surface glycoprotein CD3 gamma chain; T-cell receptor T3 gamma chain; CD3g; CD3G; T3G; CD3-γ; CD3 γ; CD3γ

• Swiss Prot:

  P09693

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthetic peptide corresponding to residues in Human CD3G.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB: 1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 20 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CD3G polyclonal antibody detects endogenous levels of CD3G protein.

• Applications Notes:

  Western blot (WB) analysis of CD3G polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4