BRM polyclonal antibody

The Brahma protein (Brm) is an ATPase subunit of the Drosophila melanogaster Brm complex, which is highly related to the mammalian SWI/SNF chromatin-remodeling complex. Brm is a transcriptional activator of Hox genes and associates with nearly all transcriptionally active chromatin in a pattern that is non-overlapping with that of Polycomb, a repressor of Hox gene transcription. The Brm complex is an essential coactivator for the trithorax group protein Zeste, a DNA-binding activator of homeotic genes. Reduction of Brm function dramatically reduces the association of RNA polymerase II with Drosophila salivary gland chromosomes, suggesting that the chromatin remodeling activity of the Brm complex plays a general role in facilitating transcription by RNA polymerase II. Brm acts as a dominant suppressor of the rough eye phenotype that results from a hypomorphic mutation of Drosophila cyclin E by inhibiting S phase entry by acting downstream of cyclin E protein accumulation. The interaction of the Brm complex with chromatin may be modulated by BAP111, which is highly associated with the Brm complex in Drosophila embryos via an HMG domain. Brm is highly expressed in unfertilized eggs and early embryos.

9007-83-4

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Probable global transcription activator SNF2L2; ATP-dependent helicase SMARCA2; BRG1-associated factor 190B; BAF190B; Protein brahma homolog; hBRM; SNF2-alpha; SNF2-α; SNF2α; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2; SMARCA2; BAF190B; SNF2A; SNF2L2

P51531

Human, Mouse, Rat

Synthetic peptide, corresponding to amino acids 1387-1432 of Human BRM.

Unconjugated

ATP-Dependent Chromatin Remodeling Complexes

WB

WB: 1:500~1:1000

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Unmodification

~ 210 kDa

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.

BRM polyclonal antibody detects endogenous levels of BRM protein.

Western blot (WB) analysis of BRM polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:Mouse brain tissue lysate Lane3:Rat brain tissue lysate