C-Src (phospho-Y529) polyclonal antibody

• Background:

  The major translational products of each of the eight members of the Src gene family identified to date are membrane-associated tyrosine protein kinases that lack transmembrane and external amino acid sequences, thereby distinguishing this group from the receptor class of tyrosine kinases. Members of this group include c-Src, c-Yes, Fyn, Lck, Lyn, Blk and c-Fgr. The major Src gene encoded protein, c-Src (also designated pp60Src, Src p60 and proto-oncogene tyrosine protein kinase Src) is expressed in a broad range of tissue and cell types, although the highest levels of c-Src are detected in neuronal tissues and platelets. c-Src may play a role in events associated with both neuronal differentiation and maintenance of mature neuronal cell functions.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Proto-oncogene tyrosine-protein kinase Src; 2.7.10.2; Proto-oncogene c-Src; pp60c-src; p60-Src; SRC1; SRC

• Swiss Prot:

  P12931

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic phosphopeptide derived from human c-Src around the phosphorylation site of Tyrosine 529.

• Conjugation:

  Unconjugated

• Related Pathways:

  Regulation of Actin Dynamics, Tumor Angiogenesis, Regulation of Microtubule Dynamics, Inhibition of Apoptosis, G Protein-coupled Receptors Signaling to MAPK Erk

• Applications:

  WB IHC

• Dilution:

  WB: 1:500~1:1000 IHC: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Phosphorylation

• Molecular Weight:

  ~ 60 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  P-c-Src (Y529) polyclonal antibody detects endogenous levels of c-Src protein only when phosphorylated at Tyr529.

• Applications Notes:

  Western blot (WB) analysis of p-c-Src (Y529) pAb at 1:500 dilution Lane1:A375 whole cell lysate (20ug) Lane2:The Uterus tissue lysate of Mouse (20ug) Lane3:The Uterus tissue lysate of Rat (20ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4