Phospho-Src (Tyr419) polyclonal antibody

• Background:

  Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2) .

• Structure Composition:

  Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Product Name Alternative:

  SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src

• Swiss Prot:

  P12931

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthesized peptide derived from human Phospho-Src (Y419)

• Conjugation:

  Unconjugated

• Applications:

  WB, FC

• Dilution:

  WB: 1/500-1/1000 FC: 1/50-1/100

• Purity:

  Affinity Purified

• Modification:

  Phosphorylated

• Molecular Weight:

  Calculated MW: 60 kDa; Observed MW: 60 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  IgG

• Applications Notes:

  Western blot analysis of Phospho-Src in A431 lysates treated with pervanadate using Phospho-Src antibody.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4