SSB (phospho-S366) polyclonal antibody

• Background:

  Ro/SSA is a ribonucleoprotein that binds to autoantibodies in 35 to 50% of patients with SLE and in up to 97% of patients with Sjogren syndrome. The Ro/SSA particle consists of a single immunoreactive protein noncovalently bound with one of four small RNA molecules. Most anti-Ro/SSA-positive sera have antibodies not only against the immunoreactive protein, but also against an Ro/SSA protein. The genes which encode the two proteins map to human chromosomes 11p15.5 and 1q31, respectively. La/SSB is an autoimmune RNA-binding protein that plays a role in the transcription of RNA polymerase III was originally defined by its reactivity with autoantibodies from patients with Sjogren syndrome and SLE.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Autoantigen La; La; La autoantigen; La autoantigen homolog; La protein; La ribonucleoprotein; La ribonucleoprotein domain family member 3; LA; LARP3; Lupus La antigen; Lupus La protein; Lupus La protein homolog; mRNA for autoantigen; Sjoegren syndrome type B antigen; Sjoegren syndrome type B antigen; Sjogren syndrome antigen B; SS B; SS-B; SS-B/La protein; SSB

• Swiss Prot:

  P05455

• Reactivity:

  Human

• Immunogen:

  Synthetic phosphopeptide derived from human SSB around the phosphorylation site of Serine 366.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB: 1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 110% (by SDS-PAGE) .

• Modification:

  Phosphorylation

• Molecular Weight:

  ~ 52 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  P-SSB (S366) polyclonal antibody detects endogenous levels of SSB protein only when phosphorylated at Serine 366.

• Applications Notes:

  Western blot (WB) analysis of p-SSB (S366) pAb at 1:500 dilution Lane1:Myla2059 whole cell lysate (40µg) Lane2:MCF-7 whole cell lysate (40µg) Lane3:HUT78 whole cell lysate (40µg) Lane4:PC3 whole cell lysate (40µg)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4