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MSK1 (Phospho-S360) polyclonal antibody

CAT: 0384-BS67581-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS67581-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
MSK1, a mitogen and stress activated protein kinase, is activated by Erk as well as p38 MAPK in response to growth factors and cellular stress, respectively . MSK1 resembles RSK because it has two kinase domains connected by a regulatory linker region . Phosphorylation of RSK1 at Ser364 and Ser381 is critical for RSK1 activity. These sites are analogous to Ser360 and Ser376 of MSK1, which may be important for MSK1 activity as well.
CAS Number
9007-83-4
Structure Composition
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Name Alternative
MSK1; Ribosomal protein S6 kinase alpha-5; S6K-alpha-5; 90 kDa ribosomal protein S6 kinase 5; Nuclear mitogen- and stress-activated protein kinase 1; RSK-like protein kinase; RSKL
Swiss Prot
O75582
Reactivity
Human, Mouse
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MSK1 with a phosphorylation site at S360. The exact sequence is proprietary.
Conjugation
Unconjugated
Related Pathways
NF-kB Signaling, G Protein-coupled Receptors Signaling to MAPK Erk, Signaling Pathways Activating p38 MAP Kinase
Applications
WB, IHC, IF/ICC
Dilution
WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/50 - 1/200)
Purity
The antibody was purified by immunogen affinity chromatography.
Modification
Phosphorylation
Molecular Weight
~ 90 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of MSK1 with a phosphorylation site at S360 protein.
Applications Notes
Western blot analysis of MSK1 (pS360) expression in mouse liver (A), mouse muscle (B) whole cell lysates.
Host or Source
Rabbit

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