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Calmodulin (K75) polyclonal antibody

CAT: 0384-BS2989-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS2989-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
The level of intracellular calcium is tightly regulated in all eukaryotic cells. A modest increase in this level can result in a myriad of physiological responses, most of which are mediated by calmodulin (CaM), the universal calcium sensor. CaM directly modulates the activity of protein kinases and phosphatases, ion channels and nitric oxide synthetases. It is generally involved in such diverse processes as cell proliferation, endocytosis, cellular adhesion, protein turn over and smooth muscle contraction. CaM (calmodulin) is an acidic protein, 148 amino acids in length, with four helix-loop-helix calcium binding domains.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Calmodulin; CaM; CALM1; CALM; CAM; CAM1; CALM2; CAM2; CAMB; CALM3; CALML2; CAM3; CAMC; CAMIII
Swiss Prot
P62158
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 50-100 of Human Calmodulin.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 22 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Calmodulin (K75) polyclonal antibody detects endogenous levels of Calmodulin protein
Applications Notes
Western blot (WB) analysis of Calmodulin (K75) pAb at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:PC3 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:BV2 whole cell lysate (40ug)
Host or Source
Rabbit

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