C-Kit (E930) polyclonal antibody

• Background:

  C-Kit is a transmembrane tyrosine kinase encoded by the c-Kit proto oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. Mutations in c-Kit have been found to be important for tumor growth and progression in a variety of cancers including mast cell diseases, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma and lung cancer. Phosphorylation at tyrosine 721 of c-Kit allows binding and activation of PI3 kinase.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Mast/stem cell growth factor receptor Kit; SCFR; Piebald trait protein; PBT; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; p145 c-kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; CD117; KIT; SCFR

• Swiss Prot:

  P10721

• Reactivity:

  Human, Mouse

• Immunogen:

  Synthetic peptide, corresponding to amino acids 901-950 of Human c-Kit.

• Conjugation:

  Unconjugated

• Related Pathways:

  Tumor-Infiltrating Immune Cell Marker Guide

• Applications:

  WB IHC

• Dilution:

  WB: 1:500~1:1000 IHC: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 120,145 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  C-Kit (E930) polyclonal antibody detects endogenous levels of c-Kit protein.

• Applications Notes:

  Western blot (WB) analysis of c-Kit (E930) polyclonal antibody at 1:500 dilution Lane1:MCF-7 cell lysate Lane2:sp2/0 cell lysate Lane3:NIH-3T3 cell lysate

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4