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Cytochrome c polyclonal antibody

CAT: 0384-CAS75125-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-CAS75125-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to mitochondrial intermembrane space. Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47 kDa) /Apaf 1. This complex processes caspase-9 from inactive proenzyme to its active form. This event further triggers caspase-3 activation and eventually leads to apoptosis.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.
Product Name Alternative
Cytochrome c; CYCS; CYC
Swiss Prot
P99999
Reactivity
Human, Rat, Mouse
Immunogen
Recombinant protein of human Cytochrome c.
Conjugation
Unconjugated
Related Pathways
Regulation of Apoptosis, AMPK Signaling Pathway
Applications
WB IHC
Dilution
WB: 1:2000~1:5000 IHC:1:50~1:100
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 17 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Cytochrome c polyclonal antibody detects endogenous levels of Cytochrome c protein.
Applications Notes
Western blot (WB) analysis of Cytochrome c polyclonal antibody at 1:2000 dilution Lane1:The Heart tissue lysate of Mouse (40ug) Lane2:The Muscle tissue lysate of Rat (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:U-87MG whole cell lysate (40ug) Lane5:HCT116 whole cell lysate (40ug)
Host or Source
Rabbit

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