CNOT7 (N19) polyclonal antibody

• Background:

  CNOT7 (CCR4-NOT transcription complex, subunit 7), also known as CAF1 (CCR4-associated factor 1), hCAF-1 or BTG1-binding factor 1, is a member of the CAF1 family. Localizing to the nucleus, CNOT7 is ubiquitously expressed and is believed to function as a transcription factor, playing a role in a wide variety of processes. CNOT7 functions as a component of the evolutionarily conserved CCR4-NOT complex, a multi-subunit complex that participates in transcription as well as mRNA degradation. CNOT7 and other subunits of the CCR4-NOT complex play a role in the regulation of nuclear hormone receptor activities. CNOT7 directly binds to and interacts with RXR∫, TOB1, TOB2, BTG1, BTG2 and BTG3. In addition, CNOT7 knockout mice are sterile and show an increase in bone mass, suggesting an important role for CNOT7 in spermatogenesis and as a suppressor of bone mass and BMP (bone morphogenetic protein) actions in osteoblasts.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  CCR4-NOT transcription complex subunit 7; BTG1-binding factor 1; CCR4-associated factor 1; CAF-1; Caf1a

• Swiss Prot:

  Q9UIV1

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to amino acids 10-54 of Human CNOT7

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB: 1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)

• Modification:

  Unmodification

• Molecular Weight:

  ~ 32 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CNOT7 (N19) polyclonal antibody detects endogenous levels of CNOT7 protein.

• Applications Notes:

  Western blot (WB) analysis of CNOT7 (N19) Antibody at 1:1000 dilution Lane1:Myla2059 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:Hela whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:3T3-L1 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4