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PP2A-α (T301) polyclonal antibody

CAT: 0384-BS1586-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS1586-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. The PP2A family comprises subfamily members PP2Aα and PP2Aβ. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha; Replication protein C; RP-C; PPP2CA; PP2Aα; PP2A α
Swiss Prot
P67775
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 270-320 of Human PP2A-α.
Conjugation
Unconjugated
Related Pathways
Contribution of Soluble Factors to EMT
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 38 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
PP2A-α (T301) polyclonal antibody detects endogenous levels of PP2A-α protein.
Applications Notes
Western blot (WB) analysis of PP2A-α (T301) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:COS-7 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:NIH-3T3 whole cell lysate (40ug)
Host or Source
Rabbit

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