PP2A-α (Phospho-Y307) polyclonal antibody

Protein phosphatase type 2A (PP2A) is an essential protein serine/threonine phosphatase that is conserved in all eukaryotes. PP2A is a key enzyme within various signal transduction pathways as it regulates fundamental cellular activities such as DNA replication, transcription, translation, metabolism, cell cycle progression, cell division, apoptosis and development. The core enzyme consists of catalytic C and regulatory A (or PR65) subunits, with each subunit represented by α and β isoforms. Additional regulatory subunits belong to four different families of unrelated proteins. Both the B (or PR55) and B' regulatory protein families contain α, β, γ and δ isoforms, with the B' family also including an ε protein. B'' family proteins include PR72, PR130, PR59 and PR48 isoforms, while striatin (PR110) and SG2NA (PR93) are both members of the B''' regulatory protein family. These B subunits competitively bind to a shared binding site on the core A subunit. This variable array of holoenzyme components, particularly regulatory B subunits, allows PP2A to act in a diverse set of functions. PP2A function is regulated by expression, localization, holoenzyme composition and post-translational modification. Phosphorylation of PP2A at Tyr307 by Src occurs in response to EGF or insulin and results in a substantial reduction of PP2A activity. Reversible methylation on the carboxyl group of Leu309 of PP2A has been observed. Methylation alters the conformation of PP2A, as well as its localization and association with B regulatory subunits.

9007-83-4

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; EC:3.1.3.16; PP2A-alpha; Replication protein C; RP-C; PPP2CA

P67775

Human, Rat, Mouse

Synthetic phosphopeptide derived from human PP2A-α around the phosphorylation site of Tyrosine 307.

Unconjugated

Contribution of Soluble Factors to EMT

WB IF

WB: 1:500~1:1000 IF: 1:100~1:500

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Phosphorylated

~ 36 kDa

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.

PP2A-α (Phospho-Y307) polyclonal antibody detects endogenous levels of PP2A-α protein only when phosphorylated at Tyr307.

Western blot (WB) analysis of PP2A-α (Phospho-Y307) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate (30ug) Lane2:HEK293T treated with λ-phosphatase whole cell lysate (30ug) Lane3:3T3-L1 whole cell lysate (30ug) Lane4:PC12 whole cell lysate (30ug)