MLST8 polyclonal antibody

• Background:

  Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E) .

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  G protein beta subunit like; G protein beta subunit-like; Gable; GBL; GBL protein; LST8; Mammalian lethal with SEC13 protein 8; MGC111011; mLST8; Protein GbetaL; Target of rapamycin complex subunit LST8; TORC subunit LST8

• Swiss Prot:

  Q9BVC4

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding Human MLST8.

• Conjugation:

  Unconjugated

• Related Pathways:

  Glutamine Metabolism, MTOR signaling

• Applications:

  WB

• Dilution:

  WB: 1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 36 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  MLST8 polyclonal antibody detects endogenous levels of MLST8 protein.

• Applications Notes:

  Western blot (WB) analysis of MLST8 polyclonal antibody at 1:500 dilution Lane1:The Heart tissue lysate of Mouse (40ug) Lane2:The Heart tissue lysate of Rat (40ug) Lane3:HCC827 whole cell lysate (40ug) Lane4:L02 whole cell lysate (40ug) Lane5:HEK293T whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4