RAB11FIP4 polyclonal antibody

• Background:

  The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated protein (Cas9) system is an adaptive immune response defense mechanism used by archea and bacteria for the degradation of foreign genetic material. This mechanism can be repurposed for other functions, including genomic engineering for mammalian systems, such as gene knockout (KO) and gene activation. CRISPR Activation Plasmid products enable the identification and upregulation of specific genes by utilizing a D10A and N863A deactivated Cas9 (dCas9) nuclease fused to a VP64 activation domain, in conjunction with sgRNA (MS2), a target-specific sgRNA engineered to bind the MS2-P65-HSF1 fusion protein. This synergistic activation mediator (SAM) transcription activation system provides a robust system to maximize the activation of endogenous gene expression.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

• Product Name Alternative:

  Rab11 family-interacting protein 4; FIP4-Rab11; Rab11-FIP4; Arfophilin-2; RAB11FIP4; ARFO2; KIAA1821

• Swiss Prot:

  Q86YS3

• Reactivity:

  Human, Rat, Mouse

• Immunogen:

  Synthetic peptide, corresponding to Human RAB11FIP4.

• Conjugation:

  Unconjugated

• Applications:

  WB IHC

• Dilution:

  WB:1:500~1:1000 IHC:1:50~1:200

• Purity:

  The protein was purified from E.coli and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 72 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -25°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  RAB11FIP4 polyclonal antibody detects endogenous levels of RAB11FIP4 protein.

• Applications Notes:

  Western blot (WB) analysis of RAB11FIP4 polyclonal antibody at 1:500 dilution LaneA:The testis tissue lysate of Rat LaneB:The testis tissue lysate of Mouse LaneC:PC3 whole cell lysate LaneD:LOVO whole cell lysate LaneE:A549 whole cell lysate

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4