CD322 polyclonal antibody

• Background :

  Junctional adhesion molecule (JAM) is a member of the immunoglobulin superfamily expressed in tight junctions of epithelial cells and endothelial cells. It is implicated in transendothelial migration of leukocytes. JAM is constitutively expressed on circulating monocytes, neutrophils, lymphocyte subsets and platelets. The JAM family consists of JAM-A, JAM-B and JAM-C, alternatively designated JAM-1, JAM-2 and JAM-3, respectively. JAM-A localizes with F-Actin at the cell-cell contacts and at the membrane ruffles. It is involved in cell to cell adhesion through homophilic interactions and plays a role in the organization of tight junctions and modulation of leukocyte extravasation. JAM-B interacts with discrete subsets of PBLs, suggesting that it may play a role in lymphocyte trafficking. JAM-B and JAM-C proteins are binding partners; JAM-C may be a functional JAM-B receptor. Specifically, JAM-B adheres to T cells through heterotypic interactions with JAM-C. The JAM-B/JAM-C interaction may play a role in T, NK and dendritic cellular inflammation.

• CAS Number :

  9007-83-4

• Structure Composition :

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative :

  Junctional adhesion molecule B; JAM-B; Junctional adhesion molecule 2; JAM-2; Vascular endothelial junction-associated molecule; VE-JAM; CD322; JAM2; C21orf43; VEJAM; UNQ219; PRO245

• Swiss Prot :

  P57087

• Reactivity :

  Human, Mouse

• Immunogen :

  Synthetic peptide, corresponding to Human CD322.

• Conjugation :

  Unconjugated

• Applications :

  WB IHC

• Dilution :

  WB:1:500~1:1000 IHC:1:50~1:200

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification :

  Unmodification

• Molecular Weight :

  ~ 43 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  CD322 polyclonal antibody detects endogenous levels of CD322 protein.

• Applications Notes :

  Western blot (WB) analysis of CD322 polyclonal antibody at 1:500 dilution LaneA:A549 whole cell lysate LaneB:HEK293T whole cell lysate LaneC:MEF whole cell lysate

• Host or Source :

  Rabbit