CD325 polyclonal antibody

• Background:

  Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development. The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton. While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking. Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers. Research studies indicate that cancer cells have upregulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion. Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis. Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Product Name Alternative:

  Cadherin-2; CDw325; Neural cadherin; N-cadherin; CD_antigen: CD325; CDH2; CDHN; NCAD

• Swiss Prot:

  P19022

• Reactivity:

  Human, Rat

• Immunogen:

  Recombinant protein of human CD325.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB: 1:2000~1:5000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 120 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CD325 polyclonal antibody detects endogenous levels of CD325 protein.

• Applications Notes:

  Western blot (WB) analysis of CD325 polyclonal antibody at 1:2000 dilution Lane1:MCF-7 whole cell lysate (30ug) Lane2:L02 whole cell lysate (30ug) Lane3:HEK293T whole cell lysate (30ug) Lane4:C6 whole cell lysate (30ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4