Islet-2 polyclonal antibody

CAT: 0384-BS62288-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0384-BS62288-01Size:50 µL
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Background
Islet-2 (insulin gene enhancer protein ISL-2) is a 359 amino acid protein encoded by the human gene ISL2. Islet-2 is a nuclear protein that contains two N-terminal LIM domains, followed by a homeodomain and a serine/glutamine/ threonine-rich C-terminus. Islet-2 is a transcriptional factor that defines subclasses of motor neurons that segregate into columns in the spinal cord and select distinct axon pathways. Islet-1 and Islet-2 are initially expressed by all postmitotic spinal motor neurons prior to diversification of somatic and visceral neuronal fates. Somatic, but not visceral, motor neurons maintain Islet-2 expression at later embryonic stages. An early phase of Islet-2 expression by prospective visceral motor neurons of the sympathetic preganglionic motor column is critical for the emergence of complete visceral motor neuron character. Mutations that reduce or eliminate both Islet-1 and Islet-2 activity will result in pronounced defects in visceral motor neuron generation and eroded somatic motor neuron character.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Insulin gene enhancer protein ISL-2; Islet-2; ISL2
Swiss Prot
Q96A47
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to Human Islet-2.
Conjugation
Unconjugated
Applications
WB IF
Dilution
WB:1:500~1:1000 IF:1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 40 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Islet-2 polyclonal antibody detects endogenous levels of Islet-2 protein.
Applications Notes
Western blot (WB) analysis of Islet-2 polyclonal antibody at 1:500 dilution LaneA:Hela whole cell lysate LaneB:The Liver tissue lysate of Mouse LaneC:The Liver tissue lysate of Rat
Host or Source
Rabbit

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