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Caldesmon (Ab-759) polyclonal antibody

CAT: 0384-BS65240-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS65240-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Caldesmon 1 (CALD1) Homo sapiens This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca (2+) -calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca (2+) -dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
CAS Number
9007-83-4
Structure Composition
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Swiss Prot
Q05682
Reactivity
Human
Immunogen
Synthesized peptide derived from human Caldesmon
Conjugation
Unconjugated
Applications
IHC-p, IF (paraffin section)
Dilution
IHC-p 1:100-500, WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Modification
Unmodification
Molecular Weight
/
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For
Specificity
This antibody detects endogenous levels of human Caldesmon. Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in paraffin section
Applications Notes
Immunohistochemical analysis of paraffin-embedded Liomyoma. 1, Antibody was diluted at 1:200 (4° overnight) . 2, Tris-EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min) .
Host or Source
Mouse/IgG2b, Kappa

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