MPRIP polyclonal antibody

CAT: 0384-BS65152-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0384-BS65152-01Size:50 µL
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Function:Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells., similarity:Contains 1 PH domain., similarity:Contains 2 PH domains., subcellular location:Colocalizes with F-actin., subunit:Binds F-actin through its N-terminus (By similarity) . Binds RHOA, PPP1R12A/MBS and PPP1R12C/MBS85 through adjacent coiled coil domains.
CAS Number
9007-83-4
Structure Composition
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Product Name Alternative
MPRIP; KIAA0864; MRIP; RHOIP3; Myosin phosphatase Rho-interacting protein; M-RIP; Rho-interacting protein 3; RIP3; p116Rip
Swiss Prot
Q6WCQ1
Reactivity
Human, Mouse, Rat
Immunogen
Synthesized peptide derived from the Internal region of human M-RIP.
Conjugation
Unconjugated
Applications
WB, ELISA
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Modification
Unmodification
Molecular Weight
~ 115 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
M-RIP Polyclonal Antibody detects endogenous levels of M-RIP protein.
Applications Notes
Western Blot analysis of various cells using M-RIP Polyclonal Antibody
Host or Source
Rabbit

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