RASA3 Polyclonal Antibody

• Background:

  RASA3 is a inhibitory regulator of the Ras-cyclic AMP pathway and is a member of the GAP1 family of GTPase-activating proteins. It stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. RASA3 is an inositol 1,3,4,5-tetrakisphosphate-binding protein (IP4) .

• Structure Composition:

  0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Product Name Alternative:

  GAP III; GAP1 (IP4BP) ; GAP1IP4BP; GAPIII; GTPase activating protein III; Inositol 1 3 4 5 tetrakisphosphate binding protein; Inositol 1345 tetrakisphosphate binding protein; Ins P4 binding protein; Ins (1 3 4 5) P4 binding protein; Ins (1345) P4 binding protein; Ras GTPase activating protein 3; RAS p21 protein activator 3; RASA 3.RASA3_HUMAN

• Swiss Prot:

  Q14644

• Reactivity:

  Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse

• Immunogen:

  KLH conjugated synthetic peptide derived from human RASA3:501-600/834

• Conjugation:

  Unconjugated

• Applications:

  IHC-P

• Dilution:

  IHC-P=1:100-500

• Purity:

  Affinity purified by Protein A

• Modification:

  Unmodification

• Molecular Weight:

  91kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  RASA3 Polyclonal Antibody detects endogenous levels of RASA3 protein.

• Applications Notes:

  Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RASA3) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4