MDM1 Polyclonal Antibody

• Background:

  This gene encodes a nuclear protein similar to the mouse double minute 1 protein. The mouse gene is located in double minute (DM) chromatin particles, is amplified in the mouse transformed 3T3 cell line, and the encoded protein is able to bind to p53. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]

• Structure Composition:

  0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Product Name Alternative:

  FLJ95264; MDM 1; Mdm1; Mdm1 nuclear protein homolog (mouse) ; Mdm1 nuclear protein homolog; MDM1_HUMAN; Mdm4 transformed 3T3 cell double minute 1 p53 binding protein (mouse) ; Mdm4 transformed 3T3 cell double minute 1 p53 binding protein; Mouse Mdm1 nuclear protein homolog; Nuclear protein double minute 1; Nuclear protein MDM1.

• Swiss Prot:

  Q8TC05

• Reactivity:

  Human, Mouse, Rat, Pig, Horse

• Immunogen:

  KLH conjugated synthetic peptide derived from human MDM1:81-180/714

• Conjugation:

  Unconjugated

• Applications:

  IHC-P

• Dilution:

  IHC-P=1:100-500

• Purity:

  Affinity purified by Protein A

• Modification:

  Unmodification

• Molecular Weight:

  ~81 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -2°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  MDM1 Polyclonal Antibody detects endogenous levels of MDM1 protein.

• Applications Notes:

  Paraformaldehyde-fixed, paraffin embedded (Rat brain) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MDM1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4