IKB alpha Polyclonal Antibody

• Background:

  Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IKB alpha and IKB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkB and block nuclear import by masking the nuclear localisation sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IKB. Immunoblotting easily detects the hyperphosphorylated forms of IKB alpha, but not phosphorylated IKB beta. Interestingly, IKB alpha and IKB beta mediate different NFkB responses. IkB alpha appears to control more transient activation of NFkB in response to an inducer, while IKB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IKB is confirmed by immunoblotting.

• Structure Composition:

  0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Product Name Alternative:

  NFKBIA; Inhibitor of KB alpha; I kappa B alpha; I (Kappa) B (alpha) ; IkappaBalpha; IKBA; IKBalpha; MAD 3; MAD3; Major histocompatibility complex enhancer binding protein MAD3; NF kappa B inhibitor alpha; NFKBI; Nuclear factor of kappa light chain gene enhancer in B cells; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha; IKBA_HUMAN.

• Swiss Prot:

  Q9Z1E3

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  KLH conjugated synthetic peptide derived from human NFKBIA:1-120/314

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC-P, FCM

• Dilution:

  WB=1:500-2000, IHC-P=1:100-500, Flow-Cyt=1ug/test

• Purity:

  Affinity purified by Protein A

• Modification:

  Unmodification

• Molecular Weight:

  37 kD

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  IKB alpha Polyclonal Antibody detects endogenous levels of IKB alpha protein.

• Applications Notes:

  Primary: Anti-IKB alpha at 1/1000 dilution

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4