IKB alpha monoclonal antibody

• Background:

  The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins . Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB . IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified .

• Structure Composition:

  Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Product Name Alternative:

  IKBA; MAD3; NFKBI; NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3

• Swiss Prot:

  P25963

• Reactivity:

  Human

• Immunogen:

  Recombinant fusion protein of human IKB alpha. The exact sequence is proprietary.

• Conjugation:

  Unconjugated

• Related Pathways:

  Regulation of Apoptosis, B Cell Receptor Signaling, Death Receptor Signaling, MAPK Erk in Growth and Differentiation Pathway, Inhibition of Apoptosis, NF-kB Signaling, T cell receptor signaling, Toll-like Receptor signaling.

• Applications:

  WB

• Dilution:

  WB (1/500 - 1/1000)

• Purity:

  This antibody is purified through a protein G column.

• Modification:

  Unmodification

• Molecular Weight:

  ~ 35 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Recognizes endogenous levels of IKB alpha protein.

• Applications Notes:

  Western blot analysis of IKB alpha expression in Hela (A), NIH3T3 (B) whole cell lysates.

• Host or Source:

  Mouse

• CAS Number:

  9007-83-4