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Ki-67 polyclonal antibody

CAT: 0384-BS65573-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS65573-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase) . It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index) . In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.
CAS Number
9007-83-4
Structure Composition
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Product Name Alternative
Antigen identified by monoclonal antibody Ki 67; Antigen KI67; KIA; Ki67; MKI67; Proliferation related Ki 67 antigen; Antigen KI-67; KI67_MOUSE.
Swiss Prot
Q91VE6
Reactivity
Human, Mouse
Immunogen
KLH conjugated synthetic peptide derived from mouse Ki-67:1231-1330/2938
Conjugation
Unconjugated
Applications
IHC-P
Dilution
IHC-P=1:100-500 () not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Purity
Affinity purified by Protein A
Modification
Unmodification
Molecular Weight
~358kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Ki-67 polyclonal Antibody detects endogenous levels of Ki-67 protein.
Applications Notes
Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ki-67) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.
Host or Source
Rabbit

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Alternative Products

CatalogName
CAU24782-01Ki-67 Protein (Ki-67) Polyclonal Antibody
TRV-004422-01Polyclonal Antibody to Ki-67 Protein (Ki-67)
MBS8592389-01Ki-67 Polyclonal Antibody
030367Ki-67 (Antigen KI-67, MKI67, Ki67, Ki 67)

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