GAPDH (Loading Control) polyclonal antibody

CAT: 0384-BS65529-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0384-BS65529-01Size:50 µL
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Background
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 38kDa subunits.
CAS Number
9007-83-4
Structure Composition
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Product Name Alternative
38 kDa BFA-dependent ADP-ribosylation substrate; Aging-associated gene 9 protein; BARS-38; cb609; EC 1.2.1.12; G3PD; G3PDH; GAPD; Glyceraldehyde 3 phosphate dehydrogenase; Glyceraldehyde 3 phosphate dehydrogenase liver; Glyceraldehyde 3 phosphate dehydrogenase muscle; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38 component; OCT1 coactivator in S phase, 38-KD component; wu:fb33a10.
Swiss Prot
P04406
Reactivity
Human, Mouse, Rat, Rabbit
Immunogen
GAPDH protein of rabbit:
Conjugation
Unconjugated
Applications
WB, IHC-P
Dilution
WB=1:2000-10000 IHC-P=1:100-500 () not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Purity
Affinity purified by Protein A
Modification
Unmodification
Molecular Weight
~37kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
GAPDH (Loading Control) polyclonal Antibody detects endogenous levels ofGAPDH (Loading Control) protein.
Applications Notes
Anti- GAPDH at 1/300 dilution
Host or Source
Rabbit

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