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GAPDH monoclonal antibody

CAT: 0384-MB66649-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-MB66649-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue, GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis, gene expression, and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases.
CAS Number
9007-83-4
Structure Composition
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Product Name Alternative
GAPD; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH
Swiss Prot
P04406
Reactivity
Human, Mouse, Rat, Hamster, Monkey, Yeast
Immunogen
Purified recombinant human GAPDH protein fragments expressed in E.coli.
Conjugation
Unconjugated
Applications
WB
Dilution
WB (1/500 - 1/1000)
Purity
The antibody was purified by immunogen affinity chromatography.
Modification
Unmodification
Molecular Weight
~ 37 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of GAPDH protein.
Applications Notes
Western blot analysis of GAPDH expression in Hela (A), NIH3T3 (B), C6 (C), COS7 (D), CHOK1 (E), rat brain (F), Jurkat (G) whole cell lysates.
Host or Source
Mouse

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