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E2F3 polyclonal antibody

CAT: 0384-BS65525-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0384-BS65525-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner. [provided by RefSeq].
CAS Number
9007-83-4
Structure Composition
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Product Name Alternative
DKFZp686C18211; E2F 3; E2F transcription factor 3; KIAA0075; MGC104598; Transcription factor E2F 3; E2F3_HUMAN; Transcription factor E2F3; E2F-3.
Swiss Prot
O00716
Reactivity
Human, Mouse, Rat, Dog, Horse, Rabbit
Immunogen
KLH conjugated synthetic peptide derived from human E2F3:91-190/465
Conjugation
Unconjugated
Applications
IHC-P
Dilution
IHC-P=1:100-500 () not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Purity
Affinity purified by Protein A
Modification
Unmodification
Molecular Weight
~49kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
E2F3 polyclonal Antibody detects endogenous levels of E2F3 protein.
Applications Notes
Paraformaldehyde-fixed, paraffin embedded (mouse brain) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (E2F3) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C.
Host or Source
Rabbit

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