E2F3 polyclonal antibody

• Background:

  The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner. [provided by RefSeq].

• Structure Composition:

  0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Product Name Alternative:

  DKFZp686C18211; E2F 3; E2F transcription factor 3; KIAA0075; MGC104598; Transcription factor E2F 3; E2F3_HUMAN; Transcription factor E2F3; E2F-3.

• Swiss Prot:

  O00716

• Reactivity:

  Human, Mouse, Rat, Dog, Horse, Rabbit

• Immunogen:

  KLH conjugated synthetic peptide derived from human E2F3:91-190/465

• Conjugation:

  Unconjugated

• Applications:

  IHC-P

• Dilution:

  IHC-P=1:100-500 () not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.

• Purity:

  Affinity purified by Protein A

• Modification:

  Unmodification

• Molecular Weight:

  ~49kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  E2F3 polyclonal Antibody detects endogenous levels of E2F3 protein.

• Applications Notes:

  Paraformaldehyde-fixed, paraffin embedded (mouse brain) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (E2F3) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4