E2F4/E2F5 (L50/83) polyclonal antibody

• Background:

  E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcriptional regulators including E2F-2, E2F-3, E2F-4, E2F-5 and E2F-6, each of which forms heterodimers with a second protein, DP-1, forming an “active” E2F transcriptional regulatory complex.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Transcription factor E2F4; E2F-4; E2F4; Transcription factor E2F5; E2F-5; E2F5

• Swiss Prot:

  Q16254 / Q15329

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to amino acids 18-72 of Human E2F4.

• Conjugation:

  Unconjugated

• Related Pathways:

  Cell Cycle Control G1 S Checkpoint

• Applications:

  IHC

• Dilution:

  IHC: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 44 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  E2F4/E2F5 (L50/83) polyclonal antibody detects endogenous levels of E2F4/E2F5 protein.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4